小鼠脑中垂体腺苷酸环化酶激活多肽及其受体mRNA的个体发生

Ontogeny of pituitary adenylate cyclase activating polypeptide and its receptor mRNA in the mouse brain.

作者信息

Shuto Y, Uchida D, Onda H, Arimura A

机构信息

U.S.-Japan Biomedical Research Laboratories, Tulane University Hebert Center, Belle Chasse, LA 70037, USA.

出版信息

Regul Pept. 1996 Dec 3;67(2):79-83. doi: 10.1016/s0167-0115(96)00116-4.

DOI:10.1016/s0167-0115(96)00116-4

PMID:8958577

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide which was first isolated from ovine hypothalamic tissue by screening for pituitary adenylate cyclase stimulating activity. Our previous data showed that radioimmunoassayable PACAP and PACAP-binding sites were detected in the whole rat brain as early as embryonic day 14(E14). In order to understand more precisely the developmental pattern of the synthesis of PACAP and its receptors in the brain, we studied the expression of PACAP and its receptor genes in the prenatal and postnatal mouse brain using RNase protection assay. The mRNAs for both PACAP and its receptor were detected as early as 9.5 days of gestation (E9.5) in the whole head of mouse embryos. The levels of PACAP mRNA in the brain increased during the prenatal period peaking at postnatal day 0 (P0). On the other hand, the levels of PACAP receptor mRNA gradually increased after E9.5. The levels sharply increased at P6 (479.0 +/- 82.5% of P0 levels), and then fell to the P3 levels at P10. These data together with our previous study on the ontogeny of PACAP immunoreactivity and its binding sites in the rat brain support the view that PACAP plays an important regulatory role in the development of brain.

摘要

垂体腺苷酸环化酶激活多肽（PACAP）是一种神经肽，它最初是通过筛选刺激垂体腺苷酸环化酶的活性，从绵羊下丘脑组织中分离出来的。我们之前的数据表明，早在胚胎第14天（E14），在大鼠全脑中就检测到了可通过放射免疫测定的PACAP和PACAP结合位点。为了更精确地了解PACAP及其受体在脑中的合成发育模式，我们使用核糖核酸酶保护分析法研究了PACAP及其受体基因在产前和产后小鼠脑中的表达。早在妊娠第9.5天（E9.5），在小鼠胚胎的整个头部就检测到了PACAP及其受体的信使核糖核酸。脑中PACAP信使核糖核酸的水平在产前阶段升高，在出生后第0天（P0）达到峰值。另一方面，PACAP受体信使核糖核酸的水平在E9.5之后逐渐升高。在P6时急剧增加（达到P0水平的479.0 +/- 82.5%），然后在P10时降至P3水平。这些数据以及我们之前关于大鼠脑中PACAP免疫反应性及其结合位点个体发生的研究支持了PACAP在脑发育中起重要调节作用的观点。