染色质核小体核心颗粒的结构。

Structure of nucleosome core particles of chromatin.

作者信息

Finch J T, Lutter L C, Rhodes D, Brown R S, Rushton B, Levitt M, Klug A

出版信息

Nature. 1977 Sep 1;269(5623):29-36. doi: 10.1038/269029a0.

Abstract

Cystals have been obtained on nucleosome cores and analysed by X-ray diffraction and electron microscopy. The core is a flat particle of dimensions about 110 X 110 X 57 A, somewhat wedge shaped, and strongly divided into two 'layers', consistent with the DNA being wound into about 1 3/4 turns of a flat superhelix of a pitch about 28 A. The organisation of the DNA can be correlated with the results to enzyme digestion studies. A change in the screw of the DNA double helix on nucleosome formation can be deduced.

摘要

已在核小体核心上获得晶体，并通过X射线衍射和电子显微镜进行分析。核心是一个扁平颗粒，尺寸约为110×110×57埃，有点呈楔形，并强烈地分为两层，这与DNA缠绕成一个螺距约为28埃的扁平超螺旋的约1 3/4圈相一致。DNA的这种组织方式可以与酶消化研究的结果相关联。可以推断出在核小体形成时DNA双螺旋的螺旋度发生了变化。