Delbaere A, Englert Y, Bergmann P J, De Prez E, Giot J M, Deschodt-Lanckman M
Department of Gynecology and Obstetrics, Erasme Hospital, Brussels, Belgium.
Peptides. 1996;17(7):1135-8. doi: 10.1016/s0196-9781(96)00184-2.
The ovarian renin-angiotensin system is involved in various aspects of human reproduction. As immunoreactive measurement of angiotensin II (ANG II) in follicular fluid (FF) relates to several angiotensin peptides with different biological activities, HPLC was used to characterize the molecular forms of the ANG II immunoreactivity in human FF. Samples of FF, obtained from gonadotropin-stimulated patients for in vitro fertilization, were collected at the time of oocyte retrieval. The C-terminal 2-8 heptapeptide was never detected. HPLC analysis revealed for the first time that the major component of the ANG II-IR in human FF was the biologically active octapeptide ANG II.
卵巢肾素-血管紧张素系统参与人类生殖的多个方面。由于卵泡液（FF）中血管紧张素II（ANG II）的免疫反应性测量涉及几种具有不同生物活性的血管紧张素肽，因此采用高效液相色谱法（HPLC）来表征人卵泡液中ANG II免疫反应性的分子形式。从接受促性腺激素刺激以进行体外受精的患者中获取卵泡液样本，并在取卵时收集。从未检测到C末端2-8七肽。HPLC分析首次揭示，人卵泡液中ANG II免疫反应性的主要成分是具有生物活性的八肽ANG II。
