Lee J D, Wang J J, Chang J H, Chung L Y, Chen E R, Yen C M
Department of Parasitology, Kaohsiung Medical College, Taiwan, Republic of China.
J Helminthol. 1996 Sep;70(3):211-4. doi: 10.1017/s0022149x0001542x.
When C57BL/6 mice were infected with Angiostrongylus cantonensis, the percentage of T helper (CD4+) cells and T supressor (CD8+) cells in peripheral blood increased weekly until the third and seventh week respectively, and then gradually decreased. C57BL/6 mice were depleted of CD4+ and CD8+ T cells by in vivo injection of anti-CD4 and anti-CD8 monoclonal antibodies, respectively, and then infected with A. cantonensis. There were significantly more and less worms recovered in the mice depleted of CD4+ and CD8+ T cells respectively than in undepleted mice. Discrete subpopulations of T cells from mice exposed to A. cantonensis for 3 weeks or 7 weeks were adoptively transferred to syngeneic recipients which were then given a challenge infection. Protection was mediated by a CD4+ T cell population present in mice after 3 weeks of infection but was not demonstrable with cells taken 7 weeks after infection. When CD4+ T cells obtained from 3-week infected mice were mixed with 5% CD8+ T cells obtained from mice infected for 7 weeks, no significant transfer of resistance was observed. Thus, immune responses to A. cantonensis in mice were regulated by discrete subpopulations of T lymphocytes.
当C57BL/6小鼠感染广州管圆线虫后，外周血中辅助性T细胞（CD4+）和抑制性T细胞（CD8+）的百分比分别在第3周和第7周前每周增加，之后逐渐下降。分别通过体内注射抗CD4和抗CD8单克隆抗体使C57BL/6小鼠的CD4+和CD8+ T细胞耗竭，然后感染广州管圆线虫。与未耗竭的小鼠相比，CD4+和CD8+ T细胞耗竭的小鼠体内回收的虫体分别显著增多和减少。将感染广州管圆线虫3周或7周的小鼠的离散T细胞亚群过继转移至同基因受体，然后对受体进行攻击感染。感染3周后的小鼠体内存在的CD4+ T细胞群体介导了保护作用，但感染7周后获取的细胞未表现出这种保护作用。当将从感染3周的小鼠获得的CD4+ T细胞与从感染7周的小鼠获得的5% CD8+ T细胞混合时，未观察到显著的抗性转移。因此，小鼠对广州管圆线虫的免疫反应由T淋巴细胞的离散亚群调节。
