Determination of benzodiazepin-1,4 derivatives in biological material. Part I. An attempt to apply high-performance liquid chromatography (HPLC) in the determination of diazepam and nitrazepam in human serum.

The application of the high performance liquid chromatography method for the determination of diazepam and nitrazepam in serum is described. This method is highly sensitive, and specific, and rapid. Calibration curves are linear in the concentration range 1-1000 ng/ml.