Krüger C, Breunig U, Biskupek-Sigwart J, Dörr H G
Abteilung Pädiatrische Endokrinologie, Universität Erlangen-Nürnberg, Germany.
Eur J Clin Chem Clin Biochem. 1996 Nov;34(11):926-9.
After introduction of the Salivette device for saliva sampling, an increase of approximately 50 ng/l was noted normal values of salivary 17-hydroxyprogesterone in our laboratory compared with previous results obtained by collection of saliva by direct spitting. To further investigate this observation systematically, 17-hydroxyprogesterone was measured in saliva of 22 healthy adults after sampling without and with the Salivette and in 15 samples of distilled water rinsed over the cotton wool swab of the Salivette. The measurements were performed with a commercially available [125I]17-hydroxyprogesterone radioimmunoassay. All steps in assay performance were exactly the same. In both series, salivary 17-hydroxyprogesterone concentrations significantly increased by 40 ng/l (p < 0.05) with use of the Salivette. The variance of the differences covered a wide range. Our results confirmed that the use of the Salivette increased salivary 17-hydroxyprogesterone values by approximately 40 ng/l. This bias is unacceptable for the establishment of reference values, as these are in the range of 20-70 ng/l in the morning. Thus, at least with our assay, the use of the Salivette is discouraged. We speculate that the cotton wool swabs contain a material. possibly derived from the biological source of the cotton wool, which cross-reacts with the antibody in the assay or affects binding affinity.
在引入用于唾液采样的唾液采集管装置后，与之前通过直接吐痰收集唾液所获得的结果相比，我们实验室中唾液17-羟孕酮的正常数值增加了约50 ng/l。为了系统地进一步研究这一观察结果，对22名健康成年人在使用和不使用唾液采集管采样后的唾液中的17-羟孕酮进行了测量，并对15份用蒸馏水冲洗过唾液采集管的棉拭子的样本进行了测量。测量使用市售的[125I]17-羟孕酮放射免疫分析方法。分析过程中的所有步骤完全相同。在两个系列中，使用唾液采集管时唾液17-羟孕酮浓度均显著增加了40 ng/l（p<0.05）。差异的方差范围很广。我们的结果证实，使用唾液采集管会使唾液17-羟孕酮值增加约40 ng/l。这种偏差对于建立参考值来说是不可接受的，因为早晨的参考值范围在20 - 70 ng/l之间。因此，至少就我们的分析方法而言，不建议使用唾液采集管。我们推测棉拭子含有一种物质，可能源自棉绒的生物来源，它在分析中与抗体发生交叉反应或影响结合亲和力。
