Beeson D, Jacobson L, Newsom-Davis J, Vincent A
Neurosciences Group, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom.
Neurology. 1996 Dec;47(6):1552-5. doi: 10.1212/wnl.47.6.1552.
Immunoprecipitation of human acetylcholine receptor (AChR) is used in the diagnostic assay for myasthenia gravis (MG). We compared human AChR derived from TE671 cells, which express fetal-type AChR, with AChR from TE671-epsilon cells, which we have engineered to express adult-type AChR. Some low-titer MG sera distinguished strikingly between the two subtypes. Four out of seven MG sera that had equivocal titers in standard assays gave positive titers with TE671-epsilon AChR, whereas only one out of seven gave a positive titer with TE671 cells. The new cell line provides a greater concentration of adult AChR than can be obtained from normal human muscle and increases the sensitivity of the diagnostic assay.
人乙酰胆碱受体（AChR）的免疫沉淀法用于重症肌无力（MG）的诊断检测。我们将源自表达胎儿型AChR的TE671细胞的人AChR与经我们改造以表达成人型AChR的TE671-ε细胞的AChR进行了比较。一些低滴度的MG血清能显著区分这两种亚型。在标准检测中滴度不明确的七份MG血清中，有四份与TE671-ε AChR反应呈阳性滴度，而与TE671细胞反应呈阳性滴度的只有七份中的一份。这种新细胞系能提供比从正常人肌肉中获得的更高浓度的成人AChR，并提高了诊断检测的灵敏度。
