Leung F C, Saggerson E D, Clark J B
Department of Biochemistry and Molecular Biology, University College London, U.K.
Int J Dev Neurosci. 1996 Oct;14(6):701-5. doi: 10.1016/s0736-5748(96)00072-x.
The effect of GTP concentration of forskolin-stimulated adenylyl cyclase activity was examined in synaptosomal membranes from 15-day-old rats that were hypothyroid owing to administration of propylthiouracil and a low-iodine diet to the mothers during pregnancy and suckling. In membranes from the forebrain hypothyroidism abolished the overall stimulatory effect of GTP, which was seen in the euthyroid case. In membranes from the hindbrain hypothyroidism had the opposite effect in that there was an enhancement of an overall stimulatory effect of GTP. It is suggested that these findings reflect changes during early development of the brain in the expression of various G-proteins and/or the expression of different isoforms of adenylyl cyclase.
在来自15日龄大鼠的突触体膜中检测了GTP浓度对福斯高林刺激的腺苷酸环化酶活性的影响。这些大鼠由于在怀孕和哺乳期间给其母亲施用丙硫氧嘧啶和低碘饮食而患有甲状腺功能减退症。在前脑的膜中，甲状腺功能减退症消除了GTP的整体刺激作用，而在甲状腺功能正常的情况下可以观察到这种作用。在后脑的膜中，甲状腺功能减退症产生了相反的效果，即GTP的整体刺激作用增强。有人认为，这些发现反映了大脑早期发育过程中各种G蛋白表达和/或腺苷酸环化酶不同同工型表达的变化。
