Voukelatou G, Sivvas E, Zeitouyian P, Kouvelas E D
Department of Physiology, Medical School, University of Patras, Greece.
Int J Dev Neurosci. 1996 Oct;14(6):779-83. doi: 10.1016/s0736-5748(96)00037-8.
The development of kainate binding proteins in membrane preparations of chick cerebellum was studied with the novel photoaffinity ligand [3H] (2S.3S.4S)-4-[1-(4-azidobenzamidomethylethenyl)-2-carboxy-3-pyr rolidineacetic acid (ABCPA). Electrophoretic analysis revealed two major radioactive bands with apparent molecular masses of 45 and 33.5 kDa and pharmacological characteristics of high (45 kDa) and low (33.5 kDa) affinity kainate binding sites. The 33.5 kDa polypeptide is present already at embryonic day (E) 13, and the amount of incorporated radioactivity remains practically constant until post-hatching day (P) 48. In contrast, the 45 kDa polypeptide is undetectable at E13. Its appearance takes place at E17, and the amount of incorporated radioactivity dramatically increases until P6. Its developmental profile is identical to that observed for [3H] kainic acid reversible binding in the same tissue. It is suggested that the 33.5 kDa polypeptide, which appears in the early stages of development may participate in receptor complexes, which play an important role in developmental phenomena.
利用新型光亲和配体3H-4-[1-(4-叠氮基苯甲酰胺甲基乙烯基)-2-羧基-3-吡咯烷乙酸(ABCPA)，研究了鸡小脑膜制剂中红藻氨酸结合蛋白的发育情况。电泳分析显示出两条主要的放射性条带，表观分子量分别为45 kDa和33.5 kDa，具有高亲和力(45 kDa)和低亲和力(33.5 kDa)红藻氨酸结合位点的药理学特征。33.5 kDa的多肽在胚胎第13天(E13)就已存在，并且掺入的放射性量在孵化后第48天(P48)之前几乎保持恒定。相比之下，45 kDa的多肽在E13时无法检测到。它在E17时出现，并且掺入的放射性量急剧增加，直到P6。其发育模式与在同一组织中观察到的[3H]红藻氨酸可逆结合的模式相同。有人认为，在发育早期出现的33.5 kDa多肽可能参与受体复合物，这些复合物在发育现象中起重要作用。
