Hayashi K, Ohkura N, Miki K, Osada S, Tomino Y
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Mol Cell Endocrinol. 1996 Oct 30;123(2):205-9. doi: 10.1016/s0303-7207(96)03909-3.
We recently isolated a novel nuclear receptor NOR-1, which is a member of the steroid/thyroid receptor superfamily, and belongs to the NGFI-B/Nur77 family. In the present study, we examined gene expression of NOR-1 and its closely related members in nephritis induced by anti-glomerular basement membrane (GBM) antibody. The mRNA levels for NOR-1, NGFI-B and RNR-1 increased 24 h after injection of anti-GBM antibody (day 1). Gene expression of NOR-1 and NGFI-B reached maximum levels on day 3, gradually decreased thereafter and returned to control levels on day 28. RNR-1 reached a peak on day 7, and then decreased. Renal injuries were most prominent on day 7 and persisted until day 28, indicating that NOR-1, NGFI-B and RNR-1 genes are induced during the early stage of glomerulonephritis and may be associated with the progression of glomerulonephritis. The induction of the NGFI-B gene was less remarkable than that of NOR-1 and RNR-1. In addition, administration of glucocorticoid hormone suppressed NOR-1 and RNR-1 gene expression to almost normal levels, whereas NGFI-B gene expression was not significantly repressed. These findings also suggest that the NGFI-B/Nur77 family may possess different biological roles and NGFI-B might act as a general transcription factor in cell function.
我们最近分离出一种新型核受体NOR-1，它是类固醇/甲状腺受体超家族的成员，属于NGFI-B/Nur77家族。在本研究中，我们检测了抗肾小球基底膜（GBM）抗体诱导的肾炎中NOR-1及其密切相关成员的基因表达。注射抗GBM抗体后24小时（第1天），NOR-1、NGFI-B和RNR-1的mRNA水平升高。NOR-1和NGFI-B的基因表达在第3天达到最高水平，此后逐渐下降，并在第28天恢复到对照水平。RNR-1在第7天达到峰值，然后下降。肾损伤在第7天最为明显，并持续到第28天，这表明NOR-1、NGFI-B和RNR-1基因在肾小球肾炎的早期被诱导，可能与肾小球肾炎的进展有关。NGFI-B基因的诱导不如NOR-1和RNR-1明显。此外，给予糖皮质激素可将NOR-1和RNR-1基因表达抑制到几乎正常水平，而NGFI-B基因表达未受到明显抑制。这些发现还表明，NGFI-B/Nur77家族可能具有不同的生物学作用，NGFI-B可能在细胞功能中作为一种通用转录因子发挥作用。
