铜离子（Cu2+）可显著增强大鼠结状神经节神经元中的ATP激活电流。

Cu2+ potently enhances ATP-activated current in rat nodose ganglion neurons.

作者信息

Li C, Peoples R W, Weight F F

机构信息

Laboratory of Molecular and Cellular Neurobiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892-8205, USA.

出版信息

Neurosci Lett. 1996 Nov 15;219(1):45-8. doi: 10.1016/s0304-3940(96)13186-4.

DOI:10.1016/s0304-3940(96)13186-4

PMID:8961300

Abstract

Several lines of evidence suggest a physiological role for Cu2+ in regulating nervous system function. In the present study using whole-cell patch-clamp recording, Cu2+ greatly enhanced current activated by 10 microM ATP in the majority of rat nodose ganglion neurons. The enhancement was concentration-dependent between 1 and 50 microM Cu2+, and had an EC50 of 6.1 microM. Cu2+ shifted the ATP concentration-response curve to the left in a parallel manner. However, Cu2+ did not enhance ATP-activated current in the presence of a maximally-effective concentration of Zn2+. The observations suggest that Cu2+ increases the affinity of the receptor for ATP by acting at the Zn2+ modulatory site. In addition, a subset of neurons in the nodose ganglion express ATP-gated receptor-channels that are insensitive to modulation by physiological concentrations of Cu2+, Zn2+ and protons.

摘要

多条证据表明Cu2+在调节神经系统功能方面具有生理作用。在本研究中，采用全细胞膜片钳记录技术，在大多数大鼠结状神经节神经元中，Cu2+极大地增强了由10微摩尔ATP激活的电流。这种增强在1至50微摩尔Cu2+之间呈浓度依赖性，半数有效浓度（EC50）为6.1微摩尔。Cu2+以平行方式将ATP浓度-反应曲线向左移动。然而，在存在最大有效浓度的Zn2+时，Cu2+并未增强ATP激活的电流。这些观察结果表明，Cu2+通过作用于Zn2+调节位点增加了受体对ATP的亲和力。此外，结状神经节中的一部分神经元表达对生理浓度的Cu2+、Zn2+和质子调节不敏感的ATP门控受体通道。