锌离子增强三磷酸腺苷对哺乳动物神经元的兴奋作用。
Zn2+ potentiates excitatory action of ATP on mammalian neurons.
作者信息
Li C, Peoples R W, Li Z, Weight F F
机构信息
Laboratory of Molecular and Cellular Neurobiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD 20852.
出版信息
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8264-7. doi: 10.1073/pnas.90.17.8264.
Abstract
Despite the increasing recognition that ATP is an important extracellular excitatory mediator in the nervous system, the regulation of ATP receptors is poorly understood. Because the extracellular Zn2+ concentration is regulated in a variety of biological tissues, we studied modulation of the ATP-gated cation channel by Zn2+ in mammalian neurons using the whole-cell patch-clamp technique. In approximately 73% of cells tested, the amplitude of ATP-activated membrane ion current increased up to 5-fold in the presence of micromolar concentrations of Zn2+. The characteristics of this action suggest that Zn2+ increases the apparent affinity of the receptor for ATP. In addition, Zn2+ increased membrane depolarization and action potential firing elicited by ATP. These observations suggest that Zn2+ may play a physiological role in regulating the excitatory action of ATP on mammalian neurons.
摘要
尽管人们越来越认识到三磷酸腺苷（ATP）是神经系统中一种重要的细胞外兴奋性介质，但对ATP受体的调节却知之甚少。由于多种生物组织中细胞外锌离子（Zn2+）浓度受到调节，我们使用全细胞膜片钳技术研究了Zn2+对哺乳动物神经元中ATP门控阳离子通道的调节作用。在大约73%的测试细胞中，在微摩尔浓度的Zn2+存在下，ATP激活的膜离子电流幅度增加了高达5倍。这种作用的特性表明，Zn2+增加了受体对ATP的表观亲和力。此外，Zn2+增强了由ATP引起的膜去极化和动作电位发放。这些观察结果表明，Zn2+可能在调节ATP对哺乳动物神经元的兴奋性作用中发挥生理作用。
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本文引用的文献
1
[Importance of zinc for histamine storage in mast cells].[锌对肥大细胞中组胺储存的重要性]
Int Arch Allergy Appl Immunol. 1963;22:112-23.
2
The capillary dilator substances in dry powders of spinal roots; a possible role of adenosine triphosphate in chemical transmission from nerve endings.脊髓神经根干粉中的毛细血管扩张物质；三磷酸腺苷在神经末梢化学传递中的可能作用。
J Physiol. 1954 Oct 28;126(1):124-40. doi: 10.1113/jphysiol.1954.sp005198.
3
Neuronal ATP receptors and their mechanism of action.神经元ATP受体及其作用机制。
Trends Pharmacol Sci. 1993 Feb;14(2):50-4. doi: 10.1016/0165-6147(93)90030-n.
4
Source and concentration of extracellular adenosine triphosphate during haemostasis in rats, rabbits and man.大鼠、兔和人止血过程中细胞外三磷酸腺苷的来源和浓度
J Physiol. 1984 Sep;354:419-29. doi: 10.1113/jphysiol.1984.sp015385.
5
Cotransmitters in the motor nerves of the guinea pig vas deferens: electrophysiological evidence.豚鼠输精管运动神经中的共递质：电生理证据。
Science. 1982 Nov 12;218(4573):693-5. doi: 10.1126/science.6291151.
6
Heavy metals in rat mast cell granules.大鼠肥大细胞颗粒中的重金属
Lab Invest. 1967 Dec;17(6):588-98.
7
The zinc content of rat mast cells.大鼠肥大细胞的锌含量。
Aust J Exp Biol Med Sci. 1968 Feb;46(1):119-21. doi: 10.1038/icb.1968.10.
8
An investigation by Raman spectroscopy of the base-proton dissociation of ATP in aqueous solution and the interactions of ATP with Zn++ and Mn++.通过拉曼光谱对ATP在水溶液中的碱基-质子解离以及ATP与Zn++和Mn++的相互作用进行的一项研究。
Biochem Biophys Res Commun. 1970 Oct 23;41(2):313-20. doi: 10.1016/0006-291x(70)90505-x.
9
Nuclear magnetic resonance evidence for a Zn2-ATP complex.锌离子 - 三磷酸腺苷复合物的核磁共振证据。
FEBS Lett. 1974 Feb 1;39(1):73-4. doi: 10.1016/0014-5793(74)80019-0.
10
Determination of Na + , K + , Mg 2+ , Cu 2+ , Zn 2+ , and Mn 2+ in rat brain regions.大鼠脑区中钠（Na⁺）、钾（K⁺）、镁（Mg²⁺）、铜（Cu²⁺）、锌（Zn²⁺）和锰（Mn²⁺）的测定
Can J Biochem. 1973 Jan;51(1):87-92. doi: 10.1139/o73-010.
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