氧气会提高反硝化副球菌反硝化细胞中硝酸盐的稳态水平。
Oxygen increases the steady-state level of nitrate in denitrifying cells of Paracoccus denitrificans.
作者信息
Kucera I, Kaplan P, Zeman A
机构信息
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
出版信息
FEMS Microbiol Lett. 1996 Dec 1;145(2):163-6. doi: 10.1111/j.1574-6968.1996.tb08572.x.
The levels of nitrate in denitrifying cells of Paracoccus denitrificans were determined by centrifugation through silicone oil into phosphoric acid and ion-exchange HPLC analysis of the cell lysates, using [14C]sucrose to correct for the trapped external medium. Introduction of oxygen brought about a significant upward shift in the intracellular nitrate concentration. This result calls into question the current thinking that oxygen blocks nitrate respiration primarily due to the inhibition of nitrate transport into the cell.
通过经硅油离心至磷酸中并对细胞裂解物进行离子交换高效液相色谱分析，测定了反硝化副球菌反硝化细胞中的硝酸盐水平，使用[14C]蔗糖校正捕获的外部培养基。引入氧气导致细胞内硝酸盐浓度显著向上变化。这一结果对当前认为氧气主要通过抑制硝酸盐转运进入细胞来阻断硝酸盐呼吸的观点提出了质疑。
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