Sánchez-Torres P, González-Candelas L, Ramón D
Departamento de Biotecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Valencia, Spain.
FEMS Microbiol Lett. 1996 Dec 1;145(2):189-94. doi: 10.1111/j.1574-6968.1996.tb08576.x.
A recombinant wine yeast strain has been constructed expressing the gene coding for alpha-L-arabinofuranosidase B from Aspergillus niger under the control of the yeast actin gene promoter. The protein is efficiently secreted by the recombinant yeast, allowing its purification and characterisation. The heterologous alpha-L-arabinofuranosidase B shows similar physico-chemical properties to the native enzyme. The wine produced in microvinification experiments using the recombinant yeast presents the same oenological characteristics as obtained with the untransformed strain. The alpha-L-arabinofuranosidase B protein is detected throughout the fermentation.
构建了一种重组葡萄酒酵母菌株，该菌株在酵母肌动蛋白基因启动子的控制下表达编码黑曲霉α-L-阿拉伯呋喃糖苷酶B的基因。该蛋白可被重组酵母有效分泌，便于其纯化和特性鉴定。异源α-L-阿拉伯呋喃糖苷酶B表现出与天然酶相似的物理化学性质。使用重组酵母进行微型酿酒实验所生产的葡萄酒呈现出与未转化菌株相同的酿酒学特性。在整个发酵过程中均可检测到α-L-阿拉伯呋喃糖苷酶B蛋白。
