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构建一种表达棘孢曲霉rhaA基因的转基因葡萄酒酵母菌株，该基因编码一种具有酿酒学意义的α-L-鼠李糖苷酶。

Construction of a genetically modified wine yeast strain expressing the Aspergillus aculeatus rhaA gene, encoding an alpha-L-rhamnosidase of enological interest.

作者信息

Manzanares Paloma, Orejas Margarita, Gil José Vicente, De Graaff Leo H, Visser Jaap, Ramón Daniel

机构信息

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Burjassot, 46100 Valencia, Spain.

出版信息

Appl Environ Microbiol. 2003 Dec;69(12):7558-62. doi: 10.1128/AEM.69.12.7558-7562.2003.

DOI:10.1128/AEM.69.12.7558-7562.2003
PMID:14660415
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309916/
Abstract

The Aspergillus aculeatus rhaA gene encoding an alpha-L-rhamnosidase has been expressed in both laboratory and industrial wine yeast strains. Wines produced in microvinifications, conducted using a combination of the genetically modified industrial strain expressing rhaA and another strain expressing a beta-glucosidase, show increased content mainly of the aromatic compound linalool.

摘要

编码α-L-鼠李糖苷酶的棘孢曲霉rhaA基因已在实验室和工业酿酒酵母菌株中表达。使用表达rhaA的转基因工业菌株与另一种表达β-葡萄糖苷酶的菌株相结合进行的微型酿酒所生产的葡萄酒，主要显示出芳族化合物芳樟醇的含量增加。

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