Kulakova A N, Reid K A, Larkin M J, Allen C C, Kulakov L A
Questor Centre, David Keir Building, Queen's University of Belfast, UK.
FEMS Microbiol Lett. 1996 Dec 1;145(2):227-31. doi: 10.1111/j.1574-6968.1996.tb08582.x.
Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chloroalkanes (C3-C8) is encoded by the same plasmid pRTL1. However, a different genetic element(s) is required for the dehalogenation of 3-chloropropionic acid. Two derivatives (P200 and P400) of R. rhodochrous NCIMB13064 were isolated which had acquired the ability to utilise naphthalene as sole carbon and energy source. Both strains lost the ability to utilise short-chain 1-chloroalkanes and underwent some rearrangements associated with pRTL1 plasmid.
红平红球菌NCIMB13064能够对多种卤代脂肪族化合物进行脱卤，并将其作为唯一的碳源和能源加以利用。NCIMB13064的突变体很容易分离得到，其对1-氯代烷烃的利用能力范围有所扩大。1-氯壬烷、1-氯癸烷和短链1-氯代烷烃（C3-C8）的脱卤作用由同一质粒pRTL1编码。然而，3-氯丙酸的脱卤作用需要不同的遗传元件。分离得到了红平红球菌NCIMB13064的两个衍生物（P200和P400），它们获得了将萘作为唯一碳源和能源加以利用的能力。这两个菌株都失去了利用短链1-氯代烷烃的能力，并经历了一些与pRTL1质粒相关的重排。
