Kulakova A N, Stafford T M, Larkin M J, Kulakov L A
Questor Centre, Queen's University of Belfast, United Kingdom.
Plasmid. 1995 May;33(3):208-17. doi: 10.1006/plas.1995.1022.
Rhodococcus rhodochrous NCIMB13064 can dehalogenate and use a wide range of 1-haloalkanes as sole carbon and energy source. The 1-chloroalkane degradation phenotype may be lost by cells spontaneously or after treatment with Mitomycin C. Two laboratory derivatives of the original strain exhibited differing degrees of stability of the chloroalkane degradation marker. Plasmids of approximately 100 kbp (pRTL1) and 80 kbp (pRTL2) have been found in R. rhodochrous NCIMB13064. pRTL1 was shown to be carrying at least some genes for the dehalogenation of 1-chloroalkanes with short chain lengths (C3 to C9). However, no connection was found between the utilization of 1-chloroalkanes with longer chain lengths (C12 to C18) and the presence of pRTL1. Three separate events were observed to lead to the inability of NCIMB13064 to dehalogenate the short-chain 1-chloroalkanes; the complete loss of pRTL1, the integration of pRTL1 into the chromosome, or the deletion of a 20-kbp fragment in pRTL1. High-frequency transfer of the 1-chloroalkane degradation marker associated with pRTL1 has been demonstrated in bacterial crosses between different derivatives of R. rhodochrous NCIMB13064.
红平红球菌NCIMB13064能够进行脱卤反应，并将多种1-卤代烷作为唯一的碳源和能源加以利用。1-氯代烷的降解表型可能会被细胞自发丧失，或在丝裂霉素C处理后丧失。原始菌株的两个实验室衍生菌株表现出不同程度的氯代烷降解标记稳定性。在红平红球菌NCIMB13064中发现了大小约为100 kbp的质粒（pRTL1）和80 kbp的质粒（pRTL2）。已证明pRTL1携带至少一些用于短链长度（C3至C9）的1-氯代烷脱卤的基因。然而，未发现长链长度（C12至C18）的1-氯代烷的利用与pRTL1的存在之间存在关联。观察到三个独立事件导致NCIMB13064无法对短链1-氯代烷进行脱卤；pRTL1完全丢失、pRTL1整合到染色体中或pRTL1中一个20 kbp片段的缺失。在红平红球菌NCIMB13064不同衍生菌株之间的细菌杂交中，已证明与pRTL1相关的1-氯代烷降解标记的高频转移。
