曲霉属在初级和次级代谢方面具有不同的脂肪酸合成酶。
Aspergillus has distinct fatty acid synthases for primary and secondary metabolism.
作者信息
Brown D W, Adams T H, Keller N P
机构信息
Department of Plant Pathology and Microbiology, Texas A&M University, College Station 77843, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14873-7. doi: 10.1073/pnas.93.25.14873.
Abstract
Aspergillus nidulans contains two functionally distinct fatty acid synthases (FASs): one required for primary fatty acid metabolism (FAS) and the other required for secondary metabolism (sFAS). FAS mutants require long-chain fatty acids for growth, whereas sFAS mutants grow normally but cannot synthesize sterigmatocystin (ST), a carcinogenic secondary metabolite structurally and biosynthetically related to aflatoxin. sFAS mutants regain the ability to synthesize ST when provided with hexanoic acid, supporting the model that the ST polyketide synthase uses this short-chain fatty acid as a starter unit. The characterization of both the polyketide synthase and FAS may provide novel means for modifying secondary metabolites.
摘要
构巢曲霉含有两种功能不同的脂肪酸合成酶（FAS）：一种是初级脂肪酸代谢所必需的（FAS），另一种是次级代谢所必需的（sFAS）。FAS突变体生长需要长链脂肪酸，而sFAS突变体生长正常，但不能合成柄曲霉素（ST），ST是一种与黄曲霉毒素在结构和生物合成上相关的致癌次级代谢产物。当给sFAS突变体提供己酸时，它们恢复了合成ST的能力，这支持了ST聚酮合酶使用这种短链脂肪酸作为起始单元的模型。聚酮合酶和FAS的特性研究可能为修饰次级代谢产物提供新方法。
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本文引用的文献
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Chem Biol. 1996 Jun;3(6):463-9. doi: 10.1016/s1074-5521(96)90094-0.
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