Tung K S, Ang J, Lou Y
Department of Pathology, University of Virginia, Charlottesville 22908, USA.
Am J Reprod Immunol. 1996 Mar;35(3):181-3. doi: 10.1111/j.1600-0897.1996.tb00028.x.
Autoantibodies to ZP3, a major glycoprotein of the zona pellucida (ZP) with sperm receptor function, can block sperm/oocyte interaction. However, only mice of certain major histocompatibility complex (MHC) haplotype respond to the ZP3 peptide. Moreover, ZP3-specific T cells can mediate ovarian autoimmune disease. A chimeric peptide has been designed that induces antibody to native ZP3 regardless of the MHC haplotype of the inbred mice tested. This results in reduction in fertility that is reversible. Infertility correlates well with ZP antibody titer, and the mice do not develop concomitant autoimmune oophoritis. The vaccine contains (1) a promiscuous foreign T-cell peptide capable of eliciting a T-cell response regardless of the animals' MHC haplotype, and (2) a modified native B-cell peptide of ZP3.
针对透明带（ZP）主要糖蛋白ZP3（具有精子受体功能）的自身抗体可阻断精子与卵母细胞的相互作用。然而，只有某些主要组织相容性复合体（MHC）单倍型的小鼠会对ZP3肽产生反应。此外，ZP3特异性T细胞可介导卵巢自身免疫性疾病。已设计出一种嵌合肽，无论所测试近交系小鼠的MHC单倍型如何，均可诱导产生针对天然ZP3的抗体。这会导致生育力下降，且这种下降是可逆的。不育与ZP抗体滴度密切相关，并且这些小鼠不会并发自身免疫性卵巢炎。该疫苗包含（1）一种通用的外源T细胞肽，无论动物的MHC单倍型如何，都能引发T细胞反应；（2）一种修饰的天然ZP3 B细胞肽。
