Functional tissue and developmental specificities of myofibrils and mitochondria in cardiac muscle.

To understand the factors underlying the functional differences between atrial and ventricular tissues, intrinsic properties of myofibrils and mitochondria of atrial skinned fibers were compared with those of fibers from adult or immature (1 and 2 weeks old) ventricular muscle. Isometric mechanical parameters were determined at various calcium concentrations in fibers treated with Triton X-100 to solubilize all cellular membranes. Maximal active tension and stiffness measured at pCa 4.5, as well as calcium sensitivity, were not different in adult atria and ventricles. Both force and stiffness increased in adult ventricles, while calcium sensitivity diminished in adult ventricles, compared with immature muscles. Myofibrillar contractile kinetics, assessed by the rate constant of tension fall following quick stretches, were similar in adult atria (79.7 +/- 6.9 s-1) and ventricles (72.4 +/- 6.8 s-1) and higher in adult atria and ventricles than in immature ventricles (24.1 +/- 2.3 s-1 in 1-week-old rats and 49.3 +/- 4.2 s-1 in 2-week-old rats). Sensitivity of rigor tension development to MgATP in the presence and in the absence of phosphocreatine was not markedly different in the different tissues. Mitochondrial function was assessed in saponin-skinned fibers. Tissue oxidative capacities, expressed as nmol O2.min-1.mg-1 fiber dry weight, were lower in immature ventricles and atria than in adult ventricles. Creatine failed to stimulate respiration in ventricles of young rats and in adult atria, whereas a 74 +/- 10% increase in respiration was observed in adult ventricles. Since mitochondrial creatine kinase was present in adult atria, this suggests an absence of coupling between oxidative phosphorylation and mitochondrial creatine kinase in this tissue. Thus, adult atrial tissue differs from neonatal ventricular tissue but it exhibits contractile properties similar to adult ventricular properties and differs from adult ventricle mainly in metabolic properties.