Haghighi S S, Perez-Espejo M A, Rodriguez F, Clapper A
Division of Neurosurgery, University of Missouri-Columbia, 65212, USA.
Spinal Cord. 1996 Apr;34(4):214-9. doi: 10.1038/sc.1996.40.
Many models have been developed to study spinal cord injury (SCI), such as cryogenic lesioning, hot water injury, scalpel lesioning, compressive trauma using clips, electromechanical devices, extradural cuffs, and weight-drop techniques. In this study, the radiofrequency (RF) lesion was used for inducing an experimental SCI in cats. The neuropathology was correlated with the MRI. In this model, 4 cats were injured at the thoracic spinal cord (T11-T12) with a lesion of 65 degrees C for 1 min using a micromanipulated penetrating RF electrode. The MRI of the lesions after 2, 3, 5, and 6 weeks post-injury as well as the correlative histological changes were obtained. The RF-induced lesion was discrete with little spreading across the spinal cord. There was a good correlation between the histopathology findings and the MRI. We conclude that experimental RF lesioning of the spinal cord can produce a consistent lesion with predictable histopathological changes in experimental animals. A 65 degree C injury for 1 min induced a clinical picture of an incomplete SCI. The RF lesioning should be considered as a new model to study SCI, particularly those with a penetrating component.
已经开发了许多模型来研究脊髓损伤（SCI），例如低温损伤、热水损伤、手术刀损伤、使用夹子的压迫性创伤、机电装置、硬膜外袖带和重物坠落技术。在本研究中，使用射频（RF）损伤在猫中诱导实验性SCI。将神经病理学与MRI相关联。在该模型中，使用微操纵穿透式RF电极，对4只猫的胸段脊髓（T11-T12）进行65摄氏度、持续1分钟的损伤。获取了损伤后2、3、5和6周的损伤MRI以及相关的组织学变化。RF诱导的损伤是离散的，很少扩散到脊髓。组织病理学结果与MRI之间存在良好的相关性。我们得出结论，在实验动物中，实验性RF脊髓损伤可产生具有可预测组织病理学变化的一致损伤。65摄氏度、持续1分钟的损伤导致不完全SCI的临床表现。RF损伤应被视为研究SCI的一种新模型，特别是那些具有穿透成分的损伤。
