Grouselle M, Grollier T, Feurtet-Mazel A, Ribeyre F, Boudou A
Centre de Recherche Paul Pascal, CNRS, Pessac, France.
Ecotoxicol Environ Saf. 1995 Dec;32(3):254-9. doi: 10.1006/eesa.1995.1111.
The experimental approach to the effects of the phenylurea herbicide Isoproturon (IPU), on the photosynthesis activity of leaf cells of the freshwater macrophyte Elodea densa was based on the fast induction kinetics of the PSII chlorophyll a in vivo fluorescence. FI/FP ratios determined on the induction curves exhibited a noticeable effect at 5 micrograms IPU.liter-1. They appeared to be the most reliable parameter for the quantitative evaluation of the dissociation constant of the complex "IPU/D1 protein" (Kd = 1.2 x 10(-7) M) and of the concentration of free IPU either at the thylakoid level or in the surrounding medium of the leaf epithelial cells. Total IPU bioaccumulation at the whole plant level as a function of [IPU] in the medium indicated that the D1 protein represented the main binding site for IPU in this aquatic plant species.
关于苯基脲类除草剂异丙隆（IPU）对淡水大型植物伊乐藻叶细胞光合作用活性影响的实验方法，是基于PSII叶绿素a体内荧光的快速诱导动力学。在诱导曲线上测定的FI/FP比值在5微克IPU·升⁻¹时表现出显著影响。它们似乎是定量评估“IPU/D1蛋白”复合物解离常数（Kd = 1.2×10⁻⁷ M）以及类囊体水平或叶上皮细胞周围介质中游离IPU浓度的最可靠参数。全株水平上IPU的总生物积累量作为培养基中[IPU]的函数表明，D1蛋白是这种水生植物物种中IPU的主要结合位点。
