Andoh A, Fujiyama Y, Sumiyoshi K, Bamba T
Department of Internal Medicine, Shiga University of Medical Science, Seta-Tukinowa, Japan.
Gastroenterology. 1996 Jun;110(6):1919-25. doi: 10.1053/gast.1996.v110.pm8964419.
BACKGROUND & AIMS: The complement system participates in the local immune system in various tissues. In this study, we investigated the local secretion of complement C3 into the pancreatic fluid and attempted to determine a possible biosynthetic site.
C3 protein in human pancreatic fluid was analyzed by, immunoblotting. The C3 messenger RNA (mRNA) expression in several pancreatic carcinoma cell lines was analyzed by the polymerase chain reaction and/or Northern blotting. The secretion of C3 by these pancreatic carcinoma cells was assessed by metabolic labeling and immunoprecipitation experiments.
In five samples of human pancreatic fluid, C3 was detected as a molecule composed of alpha and beta chains. C3 mRNA expression was observed in the ductal cell carcinoma lines (PANC-1 and MIA PaCa-2) but not in the acinar cell line (HPC-YO and AR-42J). C3 production in these cells was enhanced by interleukin 1 beta and tumor necrosis factor alpha at both the protein and the mRNA levels.
(1) Complement C3 is secreted into the exocrine fluids of the pancreas. (2) Ductal epithelial cells are possible biosynthetic sites for C3. (3) The proinflammatory cytokines, interleukin 1 beta and tumor necrosis factor alpha are effective stimulators of local C3 production in the pancreas.
补体系统参与多种组织的局部免疫系统。在本研究中，我们调查了补体C3在胰液中的局部分泌情况，并试图确定其可能的生物合成部位。
通过免疫印迹法分析人胰液中的C3蛋白。采用聚合酶链反应和/或Northern印迹法分析几种胰腺癌细胞系中C3信使核糖核酸（mRNA）的表达。通过代谢标记和免疫沉淀实验评估这些胰腺癌细胞分泌C3的情况。
在五份人胰液样本中，检测到C3是由α链和β链组成的分子。在导管细胞癌细胞系（PANC-1和MIA PaCa-2）中观察到C3 mRNA表达，但在腺泡细胞系（HPC-YO和AR-42J）中未观察到。白细胞介素1β和肿瘤坏死因子α在蛋白质和mRNA水平上均增强了这些细胞中C3的产生。
（1）补体C3分泌到胰腺的外分泌液中。（2）导管上皮细胞可能是C3的生物合成部位。（3）促炎细胞因子白细胞介素1β和肿瘤坏死因子α是胰腺局部C3产生的有效刺激物。
