Furman D P, Bukharina T A
Genetika. 1996 Aug;32(8):1074-9.
P-M status of 13 isofemale lines from a natural population of Drosophila melanogaster from Biisk (Altai) was determined by estimation of frequency of female gonadal sterility in diagnostic crosses. Content of P-elements in genomes was estimated by in situ hybridization. The average level of P-sensitivity was 97.3%; the average number of P-element copies per genome was 27.5. This value is significantly higher than estimates of average copy number of the P-element obtained for other populations with similar levels of P-sensitivity. No relationship between P-element copy number in the lines and level of their P-sensitivity was demonstrated. Based on the data obtained, the Altai population may be assigned to populations of M'-type.
通过在诊断杂交中估计雌性性腺不育频率,确定了来自比斯克(阿尔泰山)黑腹果蝇自然种群的13个同雌系的P-M状态。通过原位杂交估计基因组中P因子的含量。P敏感性的平均水平为97.3%;每个基因组中P因子拷贝的平均数量为27.5。该值显著高于对具有相似P敏感性水平的其他种群获得的P因子平均拷贝数估计值。未证明品系中P因子拷贝数与其P敏感性水平之间存在关系。根据获得的数据,阿尔泰山种群可归为M'型种群。