Pabon J E, Li X, Lei Z M, Sanfilippo J S, Yussman M A, Rao C V
Department of Obstetrics and Gynecology, University of Louisville Health Sciences Center, KY 40292, USA.
J Clin Endocrinol Metab. 1996 Jun;81(6):2397-400. doi: 10.1210/jcem.81.6.8964884.
It has been well documented that a significant proportion of chronic anovulatory patients have elevated levels of dehydroepiandrosterone sulfate (DHEAS) and luteinizing hormone (LH) and normal levels of adrenocortiocotropic hormone (ACTH). We tested the hypothesis that the zones of human adrenal cortex that secrete DHEAS may contain LH/human chorionic gonadotropin (hCG) receptors. In situ hybridization showed the presence of hybridization signals representing LH/hCG receptor mRNA transcripts in the zona reticularis. Immunocytochemistry demonstrated that the zona reticularis also contained LH/hCG receptor protein. The receptor transcripts and receptor protein are also present in the deeper layer of the zona fasciculata which can also secrete DHEAS. Double immunostaining revealed that LH/hCG receptors are present in the same cells that contain cytochrome P450 side chain cleavage enzyme, suggesting that the receptor containing cells are steroidogenic. These findings may potentially explain higher DHEAS levels in chronic anovulatory women who have normal ACTH and elevated LH levels.
有充分文献记载，相当一部分慢性无排卵患者硫酸脱氢表雄酮（DHEAS）和黄体生成素（LH）水平升高，而促肾上腺皮质激素（ACTH）水平正常。我们检验了一个假说，即分泌DHEAS的人类肾上腺皮质区域可能含有LH/人绒毛膜促性腺激素（hCG）受体。原位杂交显示，在网状带存在代表LH/hCG受体mRNA转录本的杂交信号。免疫细胞化学证明，网状带也含有LH/hCG受体蛋白。受体转录本和受体蛋白也存在于束状带较深层，束状带也能分泌DHEAS。双重免疫染色显示，LH/hCG受体存在于含有细胞色素P450侧链裂解酶的相同细胞中，这表明含有受体的细胞具有类固醇生成功能。这些发现可能有助于解释促肾上腺皮质激素正常而LH水平升高的慢性无排卵女性DHEAS水平较高的现象。
