从小鼠胸腺细胞中克隆编码δ阿片受体的全长cDNA的表达克隆。

Expression cloning of a full-length cDNA encoding delta opioid receptor from mouse thymocytes.

作者信息

Sedqi M, Roy S, Ramakrishnan S, Loh H H

机构信息

Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA.

出版信息

J Neuroimmunol. 1996 Apr;65(2):167-70. doi: 10.1016/0165-5728(96)00028-8.

DOI:10.1016/0165-5728(96)00028-8

PMID:8964899

Abstract

A delta opioid receptor complementary DNA (cDNA) was cloned by expression of cDNA library from activated thymocytes in Cos 7 cells. The deduced amino acid sequence of this receptor was similar to that described in the brain. As analyzed by southern blot hybridization, the delta opioid receptor transcripts are constitutively expressed in unactivated thymocytes. In addition, neither kappa nor mu opioid receptor transcripts were detected in thymocytes, suggesting tissue-specific opioid receptor gene expression in the immune system. The studies represent the first report of a full-length opioid receptor in the immune system.

摘要

通过在Cos 7细胞中表达来自活化胸腺细胞的cDNA文库，克隆出一种δ阿片受体互补DNA（cDNA）。该受体推导的氨基酸序列与在大脑中描述的相似。通过Southern印迹杂交分析，δ阿片受体转录本在未活化的胸腺细胞中组成性表达。此外，在胸腺细胞中未检测到κ或μ阿片受体转录本，提示免疫系统中存在组织特异性阿片受体基因表达。这些研究是免疫系统中全长阿片受体的首次报道。