Gabellec M M, Griffais R, Fillion G, Haour F
Unité de Pharmacologie Neuro-Immuno-Endocrinienne, Institut Pasteur, Paris, France.
J Neuroimmunol. 1996 May;66(1-2):65-70. doi: 10.1016/0165-5728(96)00021-5.
The expression of transcripts for Interleukin-1 (IL-1) type I and type II receptors (IL-1R1, IL-1R2) was investigated in the mouse brain and spleen using reverse transcription-polymerase chain reaction techniques under basal conditions and following injection of endotoxin (LPS, i.p., 4 mg/kg). Under basal conditions, mRNAs for both receptor types were found in various parts of the brain, in pituitary as well as in spleen. Following LPS stimulation, mRNA expressions were increased in all studied tissues. IL-1R1 mRNAs were predominant in the brain and pituitary while, IL-1R2 mRNAs were more abundant in the spleen. The maximal quantity of transcripts (IL-1R1, IL-1R2) was obtained 6 h after LPS injection in all studied tissues. The decrease to basal level was observed within 48 h in the brain. In the spleen, IL-1R1 mRNAs remained elevated 48 h after LPS while IL-1R2 mRNAs had already reached basal level. These results indicate a LPS-induced stimulation of IL-1 receptors mRNAs in the brain and a differential expression of IL-1R1 and IL-1R2 transcripts in brain and immune tissues.
采用逆转录-聚合酶链反应技术，在基础条件下以及腹腔注射内毒素（LPS，4mg/kg）后，研究了小鼠脑和脾脏中白细胞介素-1（IL-1）I型和II型受体（IL-1R1、IL-1R2）转录本的表达情况。在基础条件下，两种受体类型的mRNA在脑的各个部位、垂体以及脾脏中均有发现。LPS刺激后，所有研究组织中的mRNA表达均增加。IL-1R1 mRNA在脑和垂体中占主导，而IL-1R2 mRNA在脾脏中更为丰富。在所有研究组织中，LPS注射后6小时获得转录本（IL-1R1、IL-1R2）的最大量。脑中在48小时内观察到下降至基础水平。在脾脏中，LPS注射48小时后IL-1R1 mRNA仍升高，而IL-1R2 mRNA已达到基础水平。这些结果表明LPS可诱导脑内IL-1受体mRNA的刺激，并在脑和免疫组织中存在IL-1R1和IL-1R2转录本的差异表达。
