Giorgio S, Deberaldini E R, Pacheco R S, Pires M Q, Zanotti-Magalhães E M, Cordeiro N, Magalhães L A, Cintra M L, Guimarães N S, Stedile N M
Departamento de Parasitologia do Instituto de Biologia e Anatomia Patológica da Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Direção Regional de Saúde XII, Campinas, SP.
Rev Soc Bras Med Trop. 1996 Sep-Oct;29(5):419-24. doi: 10.1590/s0037-86821996000500003.
A study was carried out to identify Leishmania species involved in skin lesions of patients from Cosmópolis and Indaiatuba, State of São Paulo, Brazil. The epidemiological data of cutaneous leishmaniasis in two cities suggested a epidemic situation in 1994. The lesions were clinically characteristic of cutaneous leishmaniasis and five out six patients responded positively to Montenegro's intradermal test. The histopathology of skin lesions were characterized by two patterns: exudative-cellular reaction and exudative granulomatous reaction. The clinical and histopathological parameters suggested Leishmania (Viannia) braziliensis as the possible etiologic agent. In agreement, it was difficult to isolate and maintain the parasite in the laboratory. Characterization by in situ hybridization with kDNA amastigotes from lesions fragments confirmed that Leishmania (Viannia) braziliensis was the parasite responsible for the studied cutaneous lesions.
开展了一项研究，以鉴定巴西圣保罗州科莫波利斯市和因代亚图巴市患者皮肤病变中涉及的利什曼原虫种类。这两个城市皮肤利什曼病的流行病学数据表明1994年存在疫情。这些病变具有皮肤利什曼病的临床特征，六名患者中有五名对黑热病皮内试验呈阳性反应。皮肤病变的组织病理学特征有两种模式：渗出性细胞反应和渗出性肉芽肿反应。临床和组织病理学参数提示巴西利什曼原虫（维安尼亚利什曼原虫）可能是病原体。与此一致的是，在实验室中很难分离和保存该寄生虫。用病变片段的kDNA无鞭毛体进行原位杂交鉴定证实，巴西利什曼原虫（维安尼亚利什曼原虫）是所研究皮肤病变的病原体。
