Kolesnichenko T S, Gor'kova T G
Arkh Patol. 1996 Jul-Aug;58(4):56-61.
A comparative study was made of transplacental (in vivo) and direct (in vitro) effects of benz(a)pyrene (BP) in the organ cultures of embryonal lungs of mouse susceptible (strain A) and resistant (C57 Bl) to pulmonary blastomogenesis. The development of the epithelium squamous cell metaplasia was observed only after direct application of BP on the lung explants in vitro. Correlation between metaplasia frequency and BP dose and duration of treatment and mouse susceptibility to pulmonary blastomogenesis is established. Thus, at all BP concentrations (3.6 or 12 micrograms/ml of medium) and maximum time of treatment (15 days) the frequency of squamous cell metaplasia in the lung explants of C57 B1 mice was very low (3.0, 2.0 and 4.2%, p > 0.1) while in a strain A it was significant at the doses of 6 and 12 micrograms/ml (10.4 and 23.4%, p < 0.001). No epithelial metaplasia developed when the time of BP treatment was decreased up to 7 days.
对苯并(a)芘（BP）在对肺母细胞瘤发生敏感（A品系）和有抗性（C57 Bl品系）的小鼠胚胎肺器官培养物中的经胎盘（体内）和直接（体外）作用进行了一项比较研究。仅在体外将BP直接应用于肺外植体后才观察到上皮鳞状细胞化生的发展。建立了化生频率与BP剂量、治疗持续时间以及小鼠对肺母细胞瘤发生易感性之间的相关性。因此，在所有BP浓度（培养基中3.6或12微克/毫升）和最长治疗时间（15天）下，C57 B1小鼠肺外植体中鳞状细胞化生的频率非常低（3.0%、2.0%和4.2%，p>0.1），而在A品系中，在6和12微克/毫升剂量时则显著（10.4%和23.4%，p<0.001）。当BP治疗时间缩短至7天时，未发生上皮化生。
