Bannister A J, Kouzarides T
Wellcome/CRC Institute, Cambridge, UK.
Nature. 1996;384(6610):641-3. doi: 10.1038/384641a0.
The CBP protein acts as a transcriptional adaptor for many different transcription factors by directly contacting DNA-bound activators. One mechanism by which CBP is thought to stimulate transcription is by recruiting the histone acetyltransferase (HAT) P/CAF to the promoter. Here we show that CBP has intrinsic HAT activity. The HAT domain of CBP is adjacent to the binding site for the transcriptional activator E1A. Although E1A displaces P/CAF from CBP, it does not disrupt the CBP-associated HAT activity. Thus E1A carries HAT activity when complexed with CBP. Targeting CBP-associated HAT activity to specific promoters may therefore be a mechanism by which E1A acts as a transcriptional activator.
CBP蛋白通过直接接触与DNA结合的激活因子，作为许多不同转录因子的转录衔接子。CBP被认为刺激转录的一种机制是将组蛋白乙酰转移酶（HAT）P/CAF招募至启动子。我们在此表明CBP具有内在的HAT活性。CBP的HAT结构域毗邻转录激活因子E1A的结合位点。尽管E1A将P/CAF从CBP上置换下来，但它并不破坏与CBP相关的HAT活性。因此，E1A与CBP复合时具有HAT活性。因此，将与CBP相关的HAT活性靶向特定启动子可能是E1A作为转录激活因子发挥作用的一种机制。
