Müller A, Depner K R, Liess B
Institute of Virology, Hannover Veterinary School, Germany.
Dtsch Tierarztl Wochenschr. 1996 Nov;103(11):451-3.
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against classical swine fever virus (CSFV) based on the competition of the serum antibodies with a CSFV-specific monoclonal antibody directed against the viral glycoprotein gp 55 (E2), has been evaluated. A total of 553 sera obtained from pigs experimentally infected in groups with different pestiviruses were included in this study. The ELISA was applied to a group of sera collected from pigs prior to pestivirus inoculation and therefore expected to have no detectable CSFV neutralizing antibodies. The specificity of the ELISA was calculated to range between 93% and 98%. The sensitivity of the ELISA was determined by testing predominantly sera of pigs exposed to CSFV and exhibiting CSFV neutralizing antibodies. When the cut-off level was reduced by 10%, the average sensitivity increased from 60% to 70% for the groups of sera tested. The ELISA was able to discriminate between CSFV and bovine viral diarrhoea virus (BVDV) induced antibodies.
已对一种基于血清抗体与针对病毒糖蛋白gp 55（E2）的猪瘟病毒特异性单克隆抗体竞争的酶联免疫吸附测定（ELISA）进行了评估，用于检测抗经典猪瘟病毒（CSFV）的抗体。本研究共纳入了553份从经不同瘟病毒分组实验感染的猪身上获得的血清。ELISA应用于一组在瘟病毒接种前采集的猪血清，因此预计这些血清中无可检测到的CSFV中和抗体。ELISA的特异性经计算在93%至98%之间。ELISA的敏感性主要通过检测接触过CSFV并表现出CSFV中和抗体的猪血清来确定。当将临界值降低10%时，所检测血清组的平均敏感性从60%提高到了70%。该ELISA能够区分CSFV和牛病毒性腹泻病毒（BVDV）诱导的抗体。
