克罗地亚共和国野猪（Sus scrofa）体内针对经典猪瘟病毒和其他瘟病毒的血清抗体

Serum antibodies directed against classical swine fever virus and other pestiviruses in wild boar (Sus scrofa) in the Republic of Croatia.

作者信息

Roic B, Depner K R, Jemersic L, Lipej Z, Cajavec S, Toncic J, Lojkic M, Mihauevic Z

机构信息

Croatian Veterinary Institute, Zagreb, Croatia.

出版信息

Dtsch Tierarztl Wochenschr. 2007 Apr;114(4):145-8.

PMID:17484502

Abstract

The presence of serum antibodies directed against classical swine fever (CSF) virus and other pestiviruses among the wild boar (Sus scrofa) population in Croatia was investigated. During 2003, serum samples from 214 wild boars were collected in 10 hunting areas in the continental part of the country. The sera were examined by enzyme immunoassay (ELISA) and in the virus neutralization test (VNT). Out of 214 sera tested 111 (51.87 %) were positive by ELISA and regarding neutralising antibodies, against CSFV 75 (35.05 %) samples were positive. In the VNT with the C-strain (conventional live vaccine strain China) and the strain Uelzen were used. Samples were also tested for neutralizing antibodies against border disease virus (BDV) using the strain 137/4 and against bovine viral diarrhoea virus (BVDV) using the NADL strain. Neutralizing antibodies against the C-strain were detected in 36 sera (16.82 %), against strain Uelzen in 17 sera (7.94 %) and in 22 sera (10.28 %) against both strains. In five sera (2.33 %) neutralizing antibodies against BVDV and BDV were found.

摘要

对克罗地亚野猪（Sus scrofa）群体中针对经典猪瘟（CSF）病毒和其他瘟病毒的血清抗体存在情况进行了调查。2003年期间，在该国大陆部分的10个狩猎区采集了214头野猪的血清样本。通过酶免疫测定法（ELISA）和病毒中和试验（VNT）对血清进行检测。在检测的214份血清中，111份（51.87%）ELISA检测呈阳性，关于中和抗体，75份（35.05%）针对猪瘟病毒的样本呈阳性。在VNT中使用了C株（中国传统活疫苗株）和Uelzen株。还使用137/4株对样本进行了针对边境病病毒（BDV）的中和抗体检测，并使用NADL株对牛病毒性腹泻病毒（BVDV）进行了检测。在36份血清（16.82%）中检测到针对C株的中和抗体，在17份血清（7.94%）中检测到针对Uelzen株的中和抗体，在22份血清（10.28%）中检测到针对两种毒株的中和抗体。在5份血清（2.33%）中发现了针对牛病毒性腹泻病毒和边境病病毒的中和抗体。