Nakaoka R, Tabata Y, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
Biomaterials. 1996 Dec;17(23):2253-8. doi: 10.1016/0142-9612(96)00034-8.
The production profile of interleukin 1 (IL-1) from mouse peritoneal macrophages (M phi) was determined following their incubation with poly(DL-lactic acid) (PDLLA) granules containing ovalbumin (OVA). Upon incubation, M phi produced IL-1 at a significantly high rate compared with those incubated with OVA in the free form or OVA-free granules. A simple mixture of empty granules and free OVA exhibited the same level of IL-1 production as induced by free OVA alone. IL-1 production by the granules with a fixed OVA loading increased with an increase in their amount added to M phi. When incubated with a fixed amount of granules containing OVA of different loadings, M phi produced more IL-1 with an increase in the total OVA amount, but the IL-1 production decreased at OVA loadings higher than 10%. The presence of free OVA enhanced IL-1 production with the increased addition of empty granules, but the level induced by OVA loaded in granules was higher than that by mixtures of free OVA and empty granules, when compared at a similar OVA dose, irrespective of the absolute amount of PDLLA added. These findings indicate that the sustained release of OVA from the granules is critical to enhance the OVA-induced IL-1 production, in contrast to the OVA release accompanying a large initial burst, which reduced IL-1 production. It was concluded that the direct contact of PDLLA granules with M phi and the subsequent sustained release of OVA around M phi effectively activated M phi, resulting in enhanced IL-1 production.
在用含有卵清蛋白（OVA）的聚（DL-乳酸）（PDLLA）颗粒孵育小鼠腹腔巨噬细胞（M phi）后，测定了其白细胞介素1（IL-1）的产生情况。孵育后，与用游离形式的OVA或不含OVA的颗粒孵育的巨噬细胞相比，M phi以显著更高的速率产生IL-1。空颗粒和游离OVA的简单混合物产生的IL-1水平与单独用游离OVA诱导的水平相同。固定OVA负载量的颗粒诱导的IL-1产生随着添加到M phi中的颗粒量的增加而增加。当用固定量的含有不同负载量OVA的颗粒孵育时，M phi随着总OVA量的增加产生更多的IL-1，但当OVA负载量高于10%时，IL-1产生减少。游离OVA的存在随着空颗粒添加量的增加而增强IL-1产生，但在相似OVA剂量下比较时，颗粒中负载的OVA诱导的水平高于游离OVA和空颗粒混合物诱导的水平，与添加的PDLLA的绝对量无关。这些发现表明，与伴随大量初始爆发的OVA释放（这会降低IL-1产生）相反，颗粒中OVA的持续释放对于增强OVA诱导的IL-1产生至关重要。得出的结论是，PDLLA颗粒与M phi的直接接触以及随后M phi周围OVA的持续释放有效地激活了M phi，从而导致IL-1产生增加。
