Jiang H, Xie Y, Houston P, Stemke-Hale K, Mortensen U H, Rothstein R, Kodadek T
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.
J Biol Chem. 1996 Dec 27;271(52):33181-6. doi: 10.1074/jbc.271.52.33181.
The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae. The Rad51 protein is thought to be a yeast analogue of the Eschericia coli recA gene product and catalyzes strand exchange between homologous single- and double-stranded DNAs in vitro. RAD54 exhibits homologies to several known ATPases and is a member of the SWI2/MOT1 family. We show here that the Rad54 protein interacts with the Rad51 protein in vivo and in vitro and that the NH2-terminal 115 residues of the Rad54 protein are necessary for this interaction. Combined with previously reported results, these data imply that the Rad54 protein is part of a multiprotein yeast recombination complex.
RAD54和RAD51基因参与酿酒酵母中的基因重组和双链断裂修复。Rad51蛋白被认为是大肠杆菌recA基因产物的酵母类似物，并在体外催化同源单链和双链DNA之间的链交换。RAD54与几种已知的ATP酶具有同源性，是SWI2/MOT1家族的成员。我们在此表明，Rad54蛋白在体内和体外均与Rad51蛋白相互作用，并且Rad54蛋白的NH2末端115个残基对于这种相互作用是必需的。结合先前报道的结果，这些数据表明Rad54蛋白是多蛋白酵母重组复合体的一部分。
