Golub E I, Kovalenko O V, Gupta R C, Ward D C, Radding C M
Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA.
Nucleic Acids Res. 1997 Oct 15;25(20):4106-10. doi: 10.1093/nar/25.20.4106.
The cDNA for human protein HsRad54, which is a structural homolog of Saccharomyces cerevisiae recombination/repair protein Rad54, was cloned and expressed in Escherichia coli. As demonstrated by analysis in vitro and in vivo, HsRad54 protein interacts with human Rad51 recombinase. The interaction is mediated by the N-terminal domain of HsRad54 protein, which interacts with both free and DNA-bound HsRad51 protein.
人类蛋白HsRad54的互补DNA（cDNA）被克隆出来，该蛋白是酿酒酵母重组/修复蛋白Rad54的结构同源物，并在大肠杆菌中表达。体外和体内分析表明，HsRad54蛋白与人类Rad51重组酶相互作用。这种相互作用由HsRad54蛋白的N端结构域介导，该结构域与游离的和与DNA结合的HsRad51蛋白都能相互作用。
