Bernardini G, Rizzo A M, Gornati R, Rossi F, Berra B
Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, Italy.
Cell Biol Int. 1996 Oct;20(10):667-72. doi: 10.1006/cbir.1996.0088.
Xenopus embryos contain a considerable amount of a polysialo-ganglioside not yet fully characterized; in this paper, we will refer to it as ganglioside XI. Preliminary experiments indicate asialo-GMI as the core structure of the ganglioside XI and palmitic and oleic acid as the fatty acids of the ceramide moiety. Further analyses by comparative 2D-TLC with adult fish and chick embryo brains indicate the pentasialilated ganglioside GP1c as the possible structure of XI. In the adult Xenopus, XI characterizes the ganglioside pattern of the central nervous system while is absent in all the other tested tissues. At least two other more polar (presumably richer in sialic acid) bands are often visible under XI, both in embryos and in brain and spinal cord tissues of adult Xenopus. The persistence of polysialo-gangliosides in the brain and spinal cord of adult amphibians could serve to guarantee a proper functioning of the central nervous system at low body temperature.
非洲爪蟾胚胎含有大量尚未完全表征的多唾液酸神经节苷脂；在本文中，我们将其称为神经节苷脂XI。初步实验表明脱唾液酸GM1是神经节苷脂XI的核心结构，棕榈酸和油酸是神经酰胺部分的脂肪酸。通过与成年鱼类和鸡胚脑进行比较二维薄层层析的进一步分析表明，五唾液酸化神经节苷脂GP1c可能是XI的结构。在成年非洲爪蟾中，XI是中枢神经系统神经节苷脂模式的特征，而在所有其他测试组织中均不存在。在成年非洲爪蟾的胚胎以及脑和脊髓组织中，在XI下方通常至少还可见另外两条极性更强（可能富含唾液酸）的条带。成年两栖动物脑和脊髓中多唾液酸神经节苷脂的持续存在可能有助于保证中枢神经系统在低温下正常运作。
