Franck P, Sallerin J L, Schroeder H, Gelot M A, Nabet P
Laboratory of Biochemistry, University Hospital Center of Nancy, France.
Clin Chem. 1996 Dec;42(12):2015-20.
Fecal lipid content is usually determined by titrimetric or gravimetric methods, but these methods are time consuming and involve dangerous solvents. We have developed a new method of measuring fecal lipids by Fourier transform infrared spectrometry (FTIR) with an attenuated total reflectance accessory that is fast and requires no solvents. The spectra of stools from 4000 to 750 cm-1 were analyzed, and the lipid concentrations were measured by using a calibration curve prepared by partial least-squares analysis of data from 34 stools. The linearity of the method was tested by mixing low-lipid stools with lipid-overloaded stools to give a range of 0.5-15% lipid. The prediction residual values were -0.49-0.78% for calibrators, and -2.55-2.34% for unknown samples. There was good agreement between the fecal lipids measured by gravimetric (x) and FTIR(y) methods: y = 0.87x + 5.5. The standard error of prediction was 1.07%.
粪便脂质含量通常通过滴定法或重量法测定，但这些方法耗时且涉及危险溶剂。我们开发了一种通过傅里叶变换红外光谱法（FTIR）结合衰减全反射附件来测量粪便脂质的新方法，该方法快速且无需使用溶剂。分析了4000至750厘米-1范围内粪便的光谱，并通过使用对34份粪便数据进行偏最小二乘分析制备的校准曲线来测量脂质浓度。通过将低脂粪便与脂质过载粪便混合以提供0.5 - 15%的脂质范围来测试该方法的线性。校准物的预测残差值为-0.49 - 0.78%，未知样品的预测残差值为-2.55 - 2.34%。重量法（x）和FTIR法（y）测量的粪便脂质之间具有良好的一致性：y = 0.87x + 5.5。预测标准误差为1.07%。
