Kola I, Sumarsono S H
Institute of Reproduction and Development, Monash University, Clayton, Vic., Australia.
Mol Biotechnol. 1996 Oct;6(2):191-9. doi: 10.1007/BF02740773.
Mouse oocytes have proven useful in experiments aimed at studying gene function. They have been used to analyze the gain-of-function acquired after microinjection of RNA transcribed in vitro from specific gene constructs, and for establishing loss-of-function mutation obtained by injecting in vitro transcribed antisense RNA and/or synthetic oligonucleotides. This article presents protocols utilized in these studies. Specifically, the acquisition of mouse oocytes and/or embryos, the genesis of the necessary DNA and/or RNA to be used, and procedures for microinjection.
事实证明，小鼠卵母细胞在旨在研究基因功能的实验中很有用。它们已被用于分析从特定基因构建体体外转录的RNA显微注射后获得的功能获得情况，以及用于建立通过注射体外转录的反义RNA和/或合成寡核苷酸获得的功能丧失突变。本文介绍了这些研究中使用的方案。具体来说，包括小鼠卵母细胞和/或胚胎的获取、所用必要DNA和/或RNA的生成以及显微注射程序。
