Tanaka H, Honma S, Abe S, Tamura H
Third Department of Internal Medicine, Sapporo Medical University School of Medicine, Japan.
Am J Respir Crit Care Med. 1996 Dec;154(6 Pt 1):1908-12. doi: 10.1164/ajrccm.154.6.8970385.
To elucidate the immunopathologic mechanisms of Mycoplasma pneumonia, the effects of interleukin-2 (IL-2) and cyclosporin A (CYA) on Mycoplasma pneumonia in mice were investigated. Mice were intranasally inoculated with Mycoplasma pulmonis (M. pul) and treated with IL-2, CYA, or minocycline (MINO) every day between Days 3 and 9. They were killed at Days 7, 14, or 21 after the inoculation. Cell-mediated immunity (CMI) of the host was assessed by delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC). Peribronchial and perivascular lymphocyte cuffing and the accumulation of macrophages at the ends of bronchioles were exacerbated (p < 0.05) in IL-2-treated mice at Day 14 and reduced (p < 0.05) in CYA-treated mice at Day 7. Although the DTH responses to SRBC of saline-inoculated, IL-2-treated mice were increased at Days 7 (p < 0.01) and 14 (p < 0.05), those of M. pul-inoculated, IL-2-treated mice at Day 7 could not recover to the control level. The CMI levels of M. pul-inoculated, CYA-treated mice were decreased at Days 7, 14 (p < 0.05), and 21 (p < 0.01). Mycoplasma organisms in the lung showed the greatest decrease (p < 0.05) in MINO- and IL-2-treated mice, but increased at Day 21 (p < 0.05) in mice treated with IL-2 alone. These results suggest that the pathologic features of Mycoplasma pneumonia could be modified by the degree of host CMI.
为阐明支原体肺炎的免疫病理机制，研究了白细胞介素-2（IL-2）和环孢素A（CYA）对小鼠支原体肺炎的影响。将小鼠经鼻接种肺支原体（M. pul），并在第3天至第9天每天用IL-2、CYA或米诺环素（MINO）进行治疗。在接种后第7天、14天或21天处死小鼠。通过对绵羊红细胞（SRBC）的迟发型超敏反应（DTH）评估宿主的细胞介导免疫（CMI）。在第14天，IL-2治疗的小鼠支气管周围和血管周围淋巴细胞套叠以及细支气管末端巨噬细胞的积聚加剧（p<0.05），而在第7天，CYA治疗的小鼠则减少（p<0.05）。尽管在第7天（p<0.01）和第14天（p<0.05），接种生理盐水并经IL-2治疗的小鼠对SRBC的DTH反应增强，但接种M. pul并经IL-2治疗的小鼠在第7天的反应未能恢复到对照水平。接种M. pul并经CYA治疗的小鼠在第7天、14天（p<0.05）和21天（p<0.01）的CMI水平降低。肺中的支原体在MINO和IL-2治疗的小鼠中减少最多（p<0.05），但仅用IL-2治疗的小鼠在第21天增加（p<0.05）。这些结果表明，宿主CMI的程度可改变支原体肺炎的病理特征。
