人类6号和11号染色体编码的因子在A9细胞中支持1型人类免疫缺陷病毒的Rev功能。
Human chromosome 6- and 11-encoded factors support human immunodeficiency virus type 1 Rev function in A9 cells.
作者信息
Shukla R R, Marques S M, Kimmel P L, Kumar A
机构信息
Department of Biochemistry, The George Washington University Medical Center, Washington, D.C. 20037, USA.
出版信息
J Virol. 1996 Dec;70(12):9064-8. doi: 10.1128/JVI.70.12.9064-9068.1996.
Abstract
The precise mechanism of Rev-mediated expression of human immunodeficiency virus (HIV-1) late genes is not well characterized. We recently proposed a requirement for HIV-1 Rev responsive element (RRE) RNA binding host nuclear proteins in Rev function. In this report, using a transient transfection assay of Rev function, we further demonstrate the role of host cell factors in HIV-1 Rev function. Murine A9 cells, which are inefficient in forming RRE-host protein ribonucleoprotein complexes, are also inefficient in supporting Rev function. We also show that host cell factor(s) encoded by human chromosomes 6 and 11 can support HIV-1 Rev-mediated expression of unspliced viral mRNAs in murine A9 cells.
摘要
Rev介导的人类免疫缺陷病毒（HIV-1）晚期基因表达的确切机制尚未得到充分表征。我们最近提出，HIV-1 Rev反应元件（RRE）RNA结合宿主核蛋白是Rev功能所必需的。在本报告中，我们使用Rev功能的瞬时转染试验，进一步证明了宿主细胞因子在HIV-1 Rev功能中的作用。小鼠A9细胞在形成RRE-宿主蛋白核糖核蛋白复合物方面效率低下，在支持Rev功能方面也效率低下。我们还表明，人类6号和11号染色体编码的宿主细胞因子可以支持HIV-1 Rev介导的未剪接病毒mRNA在小鼠A9细胞中的表达。
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