Shmakov A N, Savidge T C
Department of Cellular Physiology, Babraham Institute, Cambridge, UK.
Epithelial Cell Biol. 1995;4(3):104-12.
The present study investigates the spatial organisation of epithelial cell proliferation in human small intestinal xenografts, in order that direct comparisons can be made with paediatric small bowel. For this purpose we employed the MIB-1 (Ki-67) monoclonal antibody and [3H]thymidine to analyse the crypt growth fraction and DNA synthesising (S-phase) cells, respectively. The spatial distribution of cycling (MIB-1+) cells was appropriately confined to the xenograft crypts where it closely resembled that of paediatric intestine, both in terms of the labelling index and an ability to form runs of labelled cells, thereby demonstrating synchronous patterns of cell division. In addition, the S-phase representation in xenograft intestine was uniform throughout the crypt proliferation compartment thereby indicating cell-cycle homogeneity. This chimeric model system now provides a new approach to investigate altered proliferative responses of human gut to a number of potentially harmful substances e.g. carcinogens, the assessment of which is not feasible in patients or volunteers.
本研究调查了人小肠异种移植中上皮细胞增殖的空间组织，以便能与小儿小肠进行直接比较。为此，我们使用MIB-1（Ki-67）单克隆抗体和[3H]胸腺嘧啶核苷分别分析隐窝生长分数和DNA合成（S期）细胞。循环（MIB-1+）细胞的空间分布恰当地局限于异种移植隐窝，在标记指数和形成标记细胞链的能力方面，其与小儿肠道非常相似，从而证明了细胞分裂的同步模式。此外，异种移植肠中S期的表现形式在整个隐窝增殖区是均匀的，从而表明细胞周期的同质性。这种嵌合模型系统现在提供了一种新方法，用于研究人类肠道对多种潜在有害物质（如致癌物）的增殖反应改变，而对这些物质的评估在患者或志愿者中是不可行的。
