Richter F, Richter A, Yang K, Lipkin M
Irving Weinstein Laboratory for Gastrointestinal Cancer Prevention, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Cancer Epidemiol Biomarkers Prev. 1992 Nov-Dec;1(7):561-6.
Epithelial cell proliferation was studied in the normal colonic mucosa of 5-week-old Sprague-Dawley rats, comparing [3H]thymidine incorporation (group 1) with two newer proliferation markers, bromodeoxyuridine (group 2) and proliferating cell nuclear antigen (group 3). Microautoradiography (group 1) or immunoperoxidase assays (groups 2 and 3) were carried out. Cells were counted for positive reaction and position along 50 colonic crypt columns/animal. No significant differences were found in number or distribution of labeled epithelial cells in proliferative compartments in crypt columns of normal colonic mucosa; labeled cells were mainly in the lower 60% of colonic crypts. Thus, in this model, bromodeoxyuridine and proliferating cell nuclear antigens were comparable to [3H]thymidine as reliable markers of proliferating epithelial cells in rat colon.
在5周龄的斯普拉格-道利大鼠的正常结肠黏膜中研究上皮细胞增殖，将[³H]胸腺嘧啶核苷掺入法（第1组）与两种更新的增殖标志物——溴脱氧尿苷（第2组）和增殖细胞核抗原（第3组）进行比较。采用微放射自显影术（第1组）或免疫过氧化物酶测定法（第2组和第3组）。对每只动物沿着50个结肠隐窝柱计数阳性反应细胞及其位置。在正常结肠黏膜隐窝柱的增殖区中，标记上皮细胞的数量和分布未发现显著差异；标记细胞主要位于结肠隐窝的下60%。因此，在该模型中，溴脱氧尿苷和增殖细胞核抗原作为大鼠结肠增殖上皮细胞的可靠标志物与[³H]胸腺嘧啶核苷相当。
