Daly J G, Kew A K, Moore A R, Olivier G
Halifax Fisheries Research Laboratory, Department of Fisheries and Oceans, NS, Canada.
Microb Pathog. 1996 Dec;21(6):447-61. doi: 10.1006/mpat.1996.0075.
Aeromonas salmonicida strains phenotypically differing in their A-layer, lipopolysaccharide, and macrophage cytotoxicity were tested in vitro for survivability in brook trout (Salvelinus fontinalis) serum with or without antibodies, and in vivo following intraperitoneal injection. The ability of brook trout peritoneal macrophages to phagocytize and kill the different phenotypes was investigated in an in vitro assay. The virulent strain, A. salmonicida 80204, which has the full complement of known virulence factors, as well as the recently described macrophage cytotoxin, was resistant in vitro to both the bactericidal activity of normal and immune serum, and to brook trout peritoneal macrophages. A. salmonicida SS-70.1, which possesses the A-layer but lacks the cytotoxin, was resistant to the bactericidal activity of normal and immune serum but was avirulent and killed by macrophages. Phenotypes lacking the A-layer, regardless of whether or not they possessed the macrophage cytotoxin were avirulent, susceptible to normal and immune serum and the bactericidal activity of peritoneal macrophages. A. salmonicida virulence expression requires both the A-layer and the macrophage cytotoxin.
对在A层、脂多糖和巨噬细胞细胞毒性方面表型不同的杀鲑气单胞菌菌株进行了体外试验，检测它们在有或没有抗体的溪红点鲑（Salvelinus fontinalis）血清中的存活能力，以及腹腔注射后的体内存活能力。在体外试验中研究了溪红点鲑腹膜巨噬细胞吞噬和杀死不同表型的能力。具有全套已知毒力因子以及最近描述的巨噬细胞细胞毒素的强毒株杀鲑气单胞菌80204，在体外对正常血清和免疫血清的杀菌活性以及溪红点鲑腹膜巨噬细胞均具有抗性。拥有A层但缺乏细胞毒素的杀鲑气单胞菌SS - 70.1对正常血清和免疫血清的杀菌活性具有抗性，但无毒且会被巨噬细胞杀死。缺乏A层的表型，无论是否拥有巨噬细胞细胞毒素，均无毒，对正常血清和免疫血清以及腹膜巨噬细胞的杀菌活性敏感。杀鲑气单胞菌的毒力表达需要A层和巨噬细胞细胞毒素。
