Schindler M, Sellers L A, Humphrey P P, Emson P C
MRC Molecular Neuroscience Group, Department of Neurobiology, Babraham Institute, Cambridge, U.K.
Neuroscience. 1997 Jan;76(1):225-40. doi: 10.1016/s0306-4522(96)00388-0.
The neuropeptide somatostatin is widely distributed in the CNS and is believed to play a role as a neurotransmitter or a neuromodulator. Somatostatin mediates its actions by the binding of the peptide to high affinity membrane receptors. The genes for five somatostatin receptor types have been cloned recently and Northern blotting and in situ hybridization studies have shown that the transcripts of all five types are expressed in the CNS. Here we report the cellular distribution of somatostatin sst2(a) receptor protein in the adult rat CNS, using a polyclonal anti-peptide antibody directed against a portion of the C-terminal domain of the receptor. The specificity of the affinity-purified antibody was demonstrated by Western blotting and immunolabelling of cells transfected with a hemagglutinin epitope-tagged version of the sst2(a) receptor. Immunohistochemistry showed a distinct distribution of the receptor protein in the rat brain. Cells and processes were labelled in a number of areas, including the basolateral amygdala, the locus coeruleus, the endopiriform nucleus, the deep layers of the cerebral cortex, the subiculum, the claustrum, the habenula, the interpenduncular nucleus, the hippocampus and the central grey. In the spinal cord, the substantia gelatinosa showed strongly-labelled cell bodies and their processes. This study provides an improved understanding of the distribution of the sst2(a) receptor in rat brain.
神经肽生长抑素广泛分布于中枢神经系统，被认为作为神经递质或神经调质发挥作用。生长抑素通过该肽与高亲和力膜受体结合来介导其作用。最近已克隆出五种生长抑素受体类型的基因，Northern印迹法和原位杂交研究表明，所有五种类型的转录本在中枢神经系统中均有表达。在此，我们使用针对受体C末端结构域一部分的多克隆抗肽抗体，报告成年大鼠中枢神经系统中生长抑素sst2(a)受体蛋白的细胞分布。通过蛋白质印迹法以及对用血凝素表位标签标记的sst2(a)受体转染的细胞进行免疫标记，证明了亲和纯化抗体的特异性。免疫组织化学显示该受体蛋白在大鼠脑中分布独特。在多个区域，包括基底外侧杏仁核、蓝斑、内梨状核、大脑皮质深层、海马下托、屏状核、缰核、脚间核、海马和中央灰质，细胞和突起均被标记。在脊髓中，胶状质显示有强标记的细胞体及其突起。本研究有助于更好地了解sst2(a)受体在大鼠脑中的分布。
