Differential dichrome staining of tissue culture monolayers: alternate dyes and possible mechanism.
作者信息
Everett M M, Miller W A
出版信息
Stain Technol. 1978 Nov;53(6):315-20. doi: 10.3109/10520297809111953.
PMID:89718
Abstract
A polyacid-dependent dichrome has been devised which will differentiate epithelial from mesenchymal cells in young dividing primary cultures. Epithelial cells and colonies and nuclei are stained with metanil yellow, the stain is fixed and differentiated with phosphotungstic acid, and the mesenchymal elements are stained with toluidine blue. Several other dyes are tested for substitution in this method. Biebrich scarlet and aniline blue could be substituted for the metanil yellow; Bismarck brown T, Janus green B, crystal violet, and neutral red could be substituted for the basic dye.
摘要
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