Romano-Silva M A, Gomez M V, Diniz C R, Cordeiro M N, Ribeiro A M
Laboratório de Neurofarmacologia-Departamento de Farmacologia, ICB Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.
Neurosci Lett. 1996 Nov 29;219(3):159-62. doi: 10.1016/s0304-3940(96)13190-6.
Fractionation of the venom of the spider Phoneutria nigriventer revealed that it was a mixture of several neurotoxic peptides. The peptides so far characterized either inhibited or induced neurotransmitter release. These effects were mediated by Ca2+ channels or increasing Na+ permeability through voltage sensitive Na(+)-channels, respectively. The pooled toxic components (fraction P4) showed stimulatory effects on acetylcholine release from brain cortical slices. In addition, a component of the observed effects resembling that of alpha-latrotoxin was identified, which was characterized by the ability to provoke release of acetylcholine (ACh) at low temperature and in a manner independent of extracellular Ca2+ and of voltage sensitive Na(+)-channels.
对巴西游走蛛毒液进行分级分离后发现，它是几种神经毒性肽的混合物。目前已鉴定出的这些肽要么抑制要么诱导神经递质释放。这些作用分别由钙通道介导，或者通过电压敏感钠通道增加钠通透性来介导。混合的毒性成分（组分P4）对脑皮质切片中乙酰胆碱的释放具有刺激作用。此外，还鉴定出一种观察到的效应类似于α- latrotoxin的成分，其特征在于能够在低温下以独立于细胞外钙和电压敏感钠通道的方式引发乙酰胆碱（ACh）的释放。
