Dobbelaar P, Mottram T, Nyabadza C, Hobbs P, Elliott-Martin R J, Schukken Y H
Department of Herd Health and Reproduction, Veterinary Faculty, Utrecht University, The Netherlands.
Vet Q. 1996 Dec;18(4):151-2. doi: 10.1080/01652176.1996.9694638.
In four healthy cows an elevation of ketone bodies was induced by reduction of feed intake. Two cows became clearly ketotic while the other two cows showed only slight increases in ketone body concentrations in serum and milk. Acetone concentrations in exhaled breath were measured by gas chromatography combined with mass spectrometry. These values were correlated with concentrations of serum beta-hydroxybutyric acid (r = 0.81) and milk acetoacetate+acetone (r = 0.70). It is concluded that the ketotic state of dairy cows can be detected by analysis of exhaled breath. This offers a potential non-invasive method of determining the metabolic state of dairy cows.
在四头健康奶牛中，通过减少采食量诱导酮体升高。两头奶牛明显出现酮血症，而另外两头奶牛血清和乳汁中的酮体浓度仅略有升高。采用气相色谱-质谱联用技术测定呼出气体中的丙酮浓度。这些值与血清β-羟基丁酸浓度（r = 0.81）和乳汁中乙酰乙酸+丙酮浓度（r = 0.70）相关。得出结论，通过分析呼出气体可检测奶牛的酮血症状态。这提供了一种潜在的非侵入性方法来确定奶牛的代谢状态。
