哺乳动物转录抑制因子RBP（CBF1）调节白细胞介素-6基因的表达。
The mammalian transcriptional repressor RBP (CBF1) regulates interleukin-6 gene expression.
作者信息
Kannabiran C, Zeng X, Vales L D
机构信息
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635, USA.
出版信息
Mol Cell Biol. 1997 Jan;17(1):1-9. doi: 10.1128/MCB.17.1.1.
Abstract
The cellular interleukin-6 (IL-6) gene contains a target site for the mammalian transcriptional repressor RBP. The target site is contained within the interleukin response element (ILRE), which mediates IL-6 activation by NF-kappa B. In this study, we show by using transient-expression assays that RBP represses activated transcription from the IL-6 gene. The presence and position of the RBP target site are crucial in mediating repression by RBP. While RBP binds within the ILRE, it does not target NF-kappa B alone; nonetheless, NF-kappa B binding to the ILRE is required for repression. Our results indicate that RBP represses coactivation by NF-kappa B and another cellular transcription factor, C/EBP-beta.
摘要
细胞白细胞介素-6（IL-6）基因包含哺乳动物转录抑制因子RBP的一个靶位点。该靶位点包含在白细胞介素反应元件（ILRE）内，ILRE介导NF-κB对IL-6的激活。在本研究中，我们通过瞬时表达分析表明，RBP抑制IL-6基因的激活转录。RBP靶位点的存在和位置对于介导RBP的抑制作用至关重要。虽然RBP结合在ILRE内，但它并非仅靶向NF-κB；尽管如此，NF-κB与ILRE的结合是抑制所必需的。我们的结果表明，RBP抑制NF-κB和另一种细胞转录因子C/EBP-β的共激活作用。
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