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AU-rich elements: characterization and importance in mRNA degradation.富含AU元件：mRNA降解中的特征与重要性
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The polyribosomal protein bound to the 3' end of histone mRNA can function in histone pre-mRNA processing.与组蛋白mRNA 3'末端结合的多核糖体蛋白可在组蛋白前体mRNA加工中发挥作用。
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Purification of a human polyribosome-associated 3' to 5' exoribonuclease.一种人源多核糖体相关的3'至5'外切核糖核酸酶的纯化
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Mammalian nonsense codons can be cis effectors of nuclear mRNA half-life.哺乳动物的无义密码子可能是细胞核mRNA半衰期的顺式效应物。
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Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.酵母MFA2基因编码的不稳定信使核糖核酸（mRNA）的去腺苷酸化会导致脱帽，随后对转录本进行5'→3'方向的消化。
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The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity.信使核糖核酸聚腺苷酸结合蛋白：定位、丰度及RNA结合特异性
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No end yet to messenger RNA 3' processing!信使核糖核酸3'端加工尚无终止！
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在体外RNA稳定性系统中，聚腺苷酸尾抑制3'至5'核酸外切酶的组装。

The poly(A) tail inhibits the assembly of a 3'-to-5' exonuclease in an in vitro RNA stability system.

作者信息

Ford L P, Bagga P S, Wilusz J

机构信息

Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark 07103, USA.

出版信息

Mol Cell Biol. 1997 Jan;17(1):398-406. doi: 10.1128/MCB.17.1.398.

DOI:10.1128/MCB.17.1.398
PMID:8972220
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC231764/
Abstract

We have developed an in vitro system which faithfully reproduces several aspects of general mRNA stability. Poly(A)- RNAs were rapidly and efficiently degraded in this system with no detectable intermediates by a highly processive 3'-to-5' exonuclease activity. The addition of a poly(A) tail of at least 30 bases, or a 3' histone stem-loop element, specifically stabilized these transcripts. Stabilization by poly(A) required the interaction of proteins with the poly(A) tail but did not apparently require a 3' OH or interaction with the 5' cap structure. Finally, movement of the poly(A) tract internal to the 3' end caused a loss of its ability to stabilize transcripts incubated in the system but did not affect its ability to interact with poly(A) binding proteins. The requirement for the poly(A) tail to be proximal to the 3' end indicates that it mediates RNA stability by blocking the assembly, but not the action, of an exonuclease involved in RNA degradation in vitro.

摘要

我们开发了一种体外系统，该系统能如实地重现一般mRNA稳定性的几个方面。在这个系统中，聚腺苷酸（Poly(A)）- RNA通过一种高度连续的3'至5'核酸外切酶活性被快速且高效地降解，且未检测到中间产物。添加至少30个碱基的聚腺苷酸尾巴或3'组蛋白茎环元件能特异性地稳定这些转录本。聚腺苷酸介导的稳定作用需要蛋白质与聚腺苷酸尾巴相互作用，但显然不需要3'羟基或与5'帽结构相互作用。最后，聚腺苷酸序列在3'端内部的移动导致其失去稳定在该系统中孵育的转录本的能力，但不影响其与聚腺苷酸结合蛋白相互作用的能力。聚腺苷酸尾巴靠近3'端的要求表明，它通过阻止参与体外RNA降解的核酸外切酶的组装而非作用来介导RNA稳定性。