Boyer J, Michaux G, Fairhead C, Gaillon L, Dujon B
Unité de Génétique Moléculaire des Levures (URA 1149 du CNRS and UFR927, Université Pierre et Marie Curie), Institut Pasteur, Paris, France.
Yeast. 1996 Dec;12(15):1575-86. doi: 10.1002/(SICI)1097-0061(199612)12:15%3C1575::AID-YEA45%3E3.0.CO;2-E.
We have determined the nucleotide sequence of a fragment of chromosome XV of Saccharomyces cerevisiae cloned into cosmid pEOA048. The analysis of the 26,857 bp sequence reveals the presence of 19 open reading frames (ORFs), and of one RNA-coding gene (SNR17A). Six ORFs correspond to previously known genes (MKK1/SSP32, YGE1/GRPE/MGE1, KIN4/KIN31/KIN3, RPL37B, DFR1 and HES1, respectively), all others were discovered in this work. Only five of the new ORFs have significant homologs in public databases, the remaining eight correspond to orphans (two of them are questionable). O5248 is a probable folypolyglutamate synthetase, having two structural homologs already sequenced in the yeast genome. O5273 shows homology with a yeast protein required for vanadate resistance. O5268 shows homology with putative oxidoreductases of different organisms. O5257 shows homology with the SAS2 protein and another hypothetical protein from yeast. The last one, O5245, shows homology with a putative protein of Caenorhabditis elegans of unknown function. The present sequence corresponds to coordinates 772,331 to 799,187 of the entire chromosome XV sequence which can be retrieved by anonymous ftp (ftp. mips. embnet. org).
我们已经确定了克隆到黏粒pEOA048中的酿酒酵母第十五号染色体片段的核苷酸序列。对26,857 bp序列的分析揭示了19个开放阅读框（ORF）和一个RNA编码基因（SNR17A）的存在。六个ORF分别对应于先前已知的基因（MKK1/SSP32、YGE1/GRPE/MGE1、KIN4/KIN31/KIN3、RPL37B、DFR1和HES1），其他所有基因都是在本研究中发现的。新的ORF中只有五个在公共数据库中有显著的同源物，其余八个对应于孤儿基因（其中两个存在疑问）。O5248可能是一种叶聚谷氨酸合成酶，在酵母基因组中已经有两个结构同源物被测序。O5273与一种抗钒酸盐所需的酵母蛋白具有同源性。O5268与不同生物体的假定氧化还原酶具有同源性。O5257与酵母的SAS2蛋白和另一种假定蛋白具有同源性。最后一个，O5245，与秀丽隐杆线虫的一种功能未知的假定蛋白具有同源性。本序列对应于整个第十五号染色体序列的772,331至799,187坐标，可通过匿名ftp（ftp.mips.embnet.org）获取。
